Publisher's Synopsis
Embryonic stem (ES) cells are important vehicles for transfer of genes into mice. When combined with normal pre-implantation embryos and transferred to foster mothers, ES cells contribute to the development of many different tissues, including the germline. When these cells are genetically manipulated, this approach discloses much about the function of specific genes.
Detailed methods for using ES cells are described in the manual Manipulating the Mouse Embryo: A Laboratory Manual, Second Edition (see page 22).
This video guide complements the above mentioned manual and Transgenic Techniques in Mice: A Video Guide (see below). It provides step-by-step demonstrations of the following procedures required for making mutant mice.
Demonstration 1: Starting embryonic stem (ES) cell lines
Demonstration 2: Making fibroblast feeder layers
Demonstration 3: Growing established ES cell lines
Demonstration 4: Targeting ES cell lines
Demonstration 5: Making chimeras with ES cells
Demonstration 6: Making a mouse
